Thomas K. Green
Research Description
Our research focuses on development of bioanalytical methods and organic synthesis. We are developing robust capillary electrophoresis (CE) with fluorescence detection for the analysis for neurotransmitters. Cyclodextrins synthesized in our own lab are used as chiral selectors to separate and detect D/L-amino acids, adenosine and sphingosine analogues. We also employ ketoreductases in stereoselective syntheses, with an aim toward synthesis of chiral fluorinated intermediates. We are also interested in developing stereoselective laboratory experiments that employ ketoreductases as biocatalysts. We have collaborated with Codexis® , a leading enzyme engineering company, to bring enzymatic catalysis into the undergraduate curriculum.
Selected Publications
1. McKee JA, Green TK “Synthesis of 2,3-O-dibenzyl-6-O-sulfobutyl-α and β cyclodextrins:
new
chiral surfactants for capillary electrophoresis” Tetrahedron Letters 2015, 56, 4451-4454.
2. Stephen TKL, Guillemette K, Green TK “Analysis of Trinitrophenylated Adenosine
and Inosine
by Capillary Electrophoresis and γ-Cyclodextrin-enhanced Fluorescence Detection”
Analytical
Chemistry 2016, 88(15), 7777-7785.
3. Green TK, Damarancha A, Vanagel M, Showalter B, Kolberg S, Thompson A “Stereoselective
Reduction of α‐Fluoro‐β‐keto Esters by NADH and NADPH‐Dependent Ketoreductases”
European Journal of Organic Chemistry 2019, 4080-4084.
Degrees
- B.S. 1977, Kearney State College
- Ph.D. 1984, University of Tennessee
Contact
Phone: 907-474-1559
Fax: 907-474-5640
Email: tkgreen@alaska.edu
Address:
Thomas K. Green
Department of Chemistry & Biochemistry
University of Alaska Fairbanks
Fairbanks, AK 99775-6160